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pet-21 vector  (Millipore)


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    Structured Review

    Millipore pet-21 vector
    Pet 21 Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet-21+vector/pet28a/pmc11323535-286-7-9
    Average 90 stars, based on 1 article reviews
    pet-21 vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Clone Assay:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Plasmid Preparation:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Expressing:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Construct:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Purification:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Polymerase Chain Reaction:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Sequencing:

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: The c DNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the c DNA of the C-terminally truncated human Endo B1 (Endo B1ΔC) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a Cterminal His6 tag (42).

    Article Title: Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
    Article Snippet: Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudocontact shifts and residual dipolar couplings.. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis.. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag.

    Article Title: Bax Activates Endophilin B1 Oligomerization and Lipid Membrane Vesiculation
    Article Snippet: Protein Expression and Purification—The cDNA of the human Endo B1 composed of 386 residues was cloned into a pET-21 vector (Novagen), and the cDNA of the C-terminally truncated human Endo B1 (Endo B1 C) comprising 290 residues was cloned into a pET-41a vector (Novagen).

    Article Title: Heterogeneous nuclear ribonucleoprotein D0 contains transactivator and DNA-binding domains
    Article Snippet: We used PCR to obtain desired fragments of the coding sequence of hnRNP D0B, and cloned them into the pET-21 vector (Novagen, Madison, WI, U.S.A.), together with a His ' tag.

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.

    Article Title: Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates
    Article Snippet: Full-length Homo sapiens laforin was subcloned into a pET-21 (Novagen) vector using NdeI and XhoI restriction sites to encode laforin and a C-terminal His 6 tag ( 42 ).

    Article Title: Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion
    Article Snippet: The amplified fragments were cloned into a pET-21 vector (Novagen), enabling cytoplasmic expression of the NadA construct with a C-terminal 6×His tag.



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